ZFIN Image: Zeng et al., 2009, Fig. 3

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Figure Caption

Fig. 3

Normalization of gata6 levels rescues loss of miR-145. (A–C) Knockdown of miR-145 results in increased expression of gata6 in zebrafish gut at 48 hpf. (D–F) Overexpression of miR-145 leads to decreased gata6 expression in the gut of 96 hpf embryos. Pericardial edema (arrowhead) is also found in miR-145 morphant. (G–I) gata6 knockdown normalizes the gut morphology (arrow) of miR-145 morphants. (J) qPCR shows strong up-regulation of gata6 in miR-145 morphants of 48 hpf. (K) qPCR indicates significantly decreased expression of gata6 in 48 hpf miR-145 mimic treated embryos. Arrows, gut; bars, mean ± SEM. *, P < 0.01. (L) The number of embryos exhibiting normal gut morphology in the rescue was analyzed by using Student′s t test (P < 0.05, n = 286). (Scale bar, 200 μm.)

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Acknowledgments

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