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Fig. S1 Phenotype after injection of miR-145 morpholino and mimic. (A) LNA in situ hybridization shows ubiquitous expression of miR-145 in uninjected wild-type embryos (UIC) and B) control MO-injected embryos. (C) miR-145 expression is significantly reduced by miR-145 MO and (D) pre-miR-145 morpholino. (E) Expression of miR-145 in uninjected or (F) control mimic expressing embryos is low in comparison to (G) miR-145 mimic-injected embryos. (H) qPCR indicates significant knocking down of miR-145 in 24 hpf embryos treated with mature miR-145 and pre-miR-145 morpholino. (I–K) Co-injection of miR-145 mimic with miR-145 morpholino can partially rescue the phenotype of miR-145 morphants. (L) pre-miR-145 MO induces a similar phenotype to miR-145 MO with gut morphological defects (arrow) and pericardial edema (arrowhead) in 96 hpf embryos. Bars, mean ± SEM. *, P < 0.05. (Scale bars, 200 μm.)