FIGURE

Fig. 3

ID

ZDB-FIG-090915-29

Publication

Maves et al., 2009 - Pbx acts with Hand2 in early myocardial differentiation

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Fig. 3

Early heart specification is normal in pbx2-MO; pbx4-MO and hand2-MO embryos. (A–O) RNA in situ expression at the 7–9 somite stage of (A–C) gata4, (D–F) gata5, (G–I) scl, (J–L) hand2, and (M–O) nkx2.5, all in blue, in (A, D, G, J, M) control, (B, E, H, K, N) pbx2-MO; pbx4-MO, or (C, F, I, L, O) hand2-MO embryos. Also included in (A–L), in red, are krox-20, labeled in (A) in r3 and r5 in the hindbrain, and myod, labeled m in (B) and used to confirm somite staging, but is not visible here in all embryos. Arrows in (M) mark nkx2.5 expression. n ≥ 13 for each marker in control, pbx2-MO; pbx4-MO, or hand2-MO. Embryos are shown in dorsal view, anterior towards the left.

Expression Data

Genes:	gata4 gata5 hand2 nkx2.5 tal1
Fish:	AB AB + MO2-hand2 AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4
Knockdown Reagents:	MO1-pbx2 MO2-hand2 MO2-pbx2 MO2-pbx4 MO3-pbx4
Anatomical Terms:	anterior lateral plate mesoderm anterior lateral plate mesoderm anterior side anterior lateral plate mesoderm posterior side anterior lateral plate mesoderm postero-medial region
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-hand2 AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4
Knockdown Reagents:	MO1-pbx2 MO2-hand2 MO2-pbx2 MO2-pbx4 MO3-pbx4
Observed In:	cardioblast cell fate specification
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 333(2), Maves, L., Tyler, A., Moens, C.B., and Tapscott, S.J., Pbx acts with Hand2 in early myocardial differentiation, 409-418, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.