ZFIN Image: Maves et al., 2009, Fig. 3

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Figure Caption

Fig. 3 Early heart specification is normal in pbx2-MO; pbx4-MO and hand2-MO embryos. (A–O) RNA in situ expression at the 7–9 somite stage of (A–C) gata4, (D–F) gata5, (G–I) scl, (J–L) hand2, and (M–O) nkx2.5, all in blue, in (A, D, G, J, M) control, (B, E, H, K, N) pbx2-MO; pbx4-MO, or (C, F, I, L, O) hand2-MO embryos. Also included in (A–L), in red, are krox-20, labeled in (A) in r3 and r5 in the hindbrain, and myod, labeled m in (B) and used to confirm somite staging, but is not visible here in all embryos. Arrows in (M) mark nkx2.5 expression. n ≥ 13 for each marker in control, pbx2-MO; pbx4-MO, or hand2-MO. Embryos are shown in dorsal view, anterior towards the left.

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Acknowledgments

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Reprinted from Developmental Biology, 333(2), Maves, L., Tyler, A., Moens, C.B., and Tapscott, S.J., Pbx acts with Hand2 in early myocardial differentiation, 409-418, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.