FIGURE

Fig. S6

ID

ZDB-FIG-080605-6

Publication

Sidi et al., 2008 - Chk1 Suppresses a Caspase-2 Apoptotic Response to DNA Damage that Bypasses p53, Bcl-2, and Caspase-3

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Fig. S6

puma depletion abrogates IR-induced apoptosis in wild-type zebrafish embryos
Lateral views of 25.5-hpf wild-type zebrafish embryos that were injected at the 1-cell stage with the indicated MOs, irradiated (0- or 12.5-Gy) at 18 hpf, and immunostained with an anti-active-caspase-3 antibody. The p53 MO-injected embryo serves as an internal control. Note that puma MO, but not puma 5bpmmMO, produces a complete phenocopy of p53 MO.

Expression Data

Antibody:	Ab1-casp3
Fish:	WT WT + MO2-bbc3 WT + MO4-tp53
Condition:	radiation
Knockdown Reagents:	MO2-bbc3 MO4-tp53
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO2-bbc3 WT + MO4-tp53
Condition:	radiation
Knockdown Reagents:	MO2-bbc3 MO4-tp53
Observed In:	apoptotic process DNA damage response whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 133(5), Sidi, S., Sanda, T., Kennedy, R.D., Hagen, A.T., Jette, C.A., Hoffmans, R., Pascual, J., Imamura, S., Kishi, S., Amatruda, J.F., Kanki, J.P., Green, D.R., D'Andrea, A.A., and Look, A.T., Chk1 Suppresses a Caspase-2 Apoptotic Response to DNA Damage that Bypasses p53, Bcl-2, and Caspase-3, 864-877, Copyright (2008) with permission from Elsevier. Full text @ Cell