Fig. S2

Melanoblast differentiation and migration do not recover at 30 hpf. All panels are lateral views of 30 hpf embryos that are stained by in situ hybridization to reveal expression of mifta (A, B), c-kit (C, D), dct (E, F), fms (G, H) and xdh (I, J). A, B: By 30 hpf, the number of melanoblasts that express mitfa does not recover in hdac1^col mutants. Also, most of the mitfa-positive melanoblasts fail to migrate and are located in the dorsal stripe and in the post otic region in hdac1^col mutants. In contrast, wild-type melanoblasts have migrated into the ventral stripe and over the head. C, D: Additionally, melanoblast-specific c-kit expression in hdac1^col is still absent and/or reduced (arrowhead), although non-melanoblast expression of c-kit in the post anal region and posterior mesoderm is equivalent to wild-type (arrows). E, F: There are fewer dct-positive differentiating melanoblasts in hdac1^col mutants as compared to wild-type (arrowheads) and most of the dct-positive melanoblasts are located posterior to the otic vesicle and in the dorsal stripe. A few dct-positive melanoblasts are migrating in the anterior trunk in hdac1^col mutants, although dct is not expressed as robustly compared to expression in wild-type melanoblasts. G, H: In contrast to c-kit-positive melanoblasts, there are many more fms-positive differentiating xanthoblasts in hdac1^col mutants compared to wild-type. I, J: Although reduced in number when compared to wild-type, xdh-positive differentiating xanthoblasts are more numerous in the cranial region (arrows) and trunk (arrowheads) as compared to differentiating dct-positive melanoblasts in hdac1^col mutants.