FIGURE

Fig. 8

ID

ZDB-FIG-080401-20

Publication

Ignatius et al., 2008 - colgate/hdac1 repression of foxd3 expression is required to permit mitfa-dependent melanogenesis

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Fig. 8

Foxd3 negatively regulates mitfa. (A–D) Lateral views of mitfa in situ hybridizations of 32 hpf embryos. (B, D) There is an increase in the number of mitfa-positive melanoblasts in hdac1^col/foxd3 mutant/morphant embryos as compared to uninjected hdac1^col mutants. (A, C) In wild-type/foxd3 morphant embryos mitfa expression in melanoblasts is more robust than uninjected controls. (E–H) Lateral views of c-kit in situ hybridizations in 32 hpf embryos. (F, H) There is no rescue of c-kit expression in melanoblasts in hdac1^col/foxd3 mutant morphants when compared to uninjected hdac1^col mutants at 32 hpf. (E, G) Qualitatively, there are fewer c-kit positive melanoblasts in foxd3 morpholino treated wild-type embryos as compared to uninjected wild-type embryos. (E–H) In contrast to melanoblast expression, there is no difference in c-kit expression in the post anal region between wild-type, hdac1^col mutants, wt/foxd3 wild-type/morphants and hdac1^col/foxd3 mutant/morphant embryos (arrows).

Expression Data

Genes:	kita mitfa
Fish:	WT hdac1^b382/b382 WT + MO3-foxd3 hdac1^b382/b382 + MO3-foxd3
Knockdown Reagent:	MO3-foxd3
Anatomical Term:	melanoblast
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 313(2), Ignatius, M.S., Moose, H.E., El-Hodiri, H.M., and Henion, P.D., colgate/hdac1 repression of foxd3 expression is required to permit mitfa-dependent melanogenesis, 568-583, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.