FIGURE

Fig. 2

ID

ZDB-FIG-071217-1

Publication

Tsujikawa et al., 2007 - Mechanism of positioning the cell nucleus in vertebrate photoreceptors

Other Figures

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Fig. 2

Molecular characterization of the mikre oko (mok) gene. (A) Positional cloning of mok. (B) A schematic diagram of the zebrafish Mok polypeptide in comparison with its Drosophila and mouse homologs. In all three species, Mok polypeptide includes domains thought to bind microtubules (MT), dynein intermediate chain (DIC), actin-related protein 1 (ARP1), and BBS protein 4 (BBS4). In both the zebrafish and the fly, mutant alleles produce C-terminal truncations of the polypeptide (arrowheads). (C) A transverse section through the retina showing mok transcript distribution as evaluated by in situ hybridization. Arrowhead indicates the photoreceptor cell layer, and the asterisk indicates the lens. (Inset) RT-PCR reveals that the mok mRNA level is severely reduced in mok^m632 mutant homozygotes, compared with their wild-type siblings. β-actin level (act) is unchanged. (D and E) Mok polypeptide distribution in zebrafish (D) and mouse (E) retinae. (Right) Anti-Mok antibody staining (red). (Left) F-actin distribution (blue). Staining is particularly prominent apical to the outer limiting membrane (arrowheads). In D and E, retinal pigmented epithelium is up.

Expression Data

Gene:	dctn1a
Fish:	AB
Anatomical Terms:	eye retinal photoreceptor layer
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA