FIGURE

Fig. 2

ID

ZDB-FIG-070620-12

Publication

Robu et al., 2007 - p53 activation by knockdown technologies

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Fig. 2

p53 MO Attenuates Apoptosis Induced by MOs and GripNAs, as Detected by TUNEL Assay (A–M) Fluorescent images of 30 hpf embryos injected with indicated MOs and subjected to TUNEL assay to detect apoptosis. A strong fluorescent signal for Smo MO (C), Wnt5 MO1 (E), and Wnt5 GripNA1 (H), similar to Mdm2 MO (L), indicates increased apoptosis. The fluorescent signal is strongly diminished by co-knockdown of p53 (D, F, I, and M), similar to the uninjected control (A), Wnt5 MO2 (G), or Wnt5 mutant (J). Wnt5 mutant is not affected by p53 knockdown (K).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wnt5b^{hi1780bTg/hi1780bTg} WT + MO1-mdm2 WT + MO1-mdm2 + MO4-tp53 WT + MO2-wnt5b WT + MO2-wnt5b + MO4-tp53 WT + MO3-wnt5b WT + MO4-smo WT + MO4-smo + MO4-tp53 WT + MO4-tp53 wnt5b^{hi1780bTg/hi1780bTg} + MO4-tp53 (all 10)
Knockdown Reagents:	MO1-mdm2 MO2-wnt5b MO3-wnt5b MO4-smo MO4-tp53
Observed In:	nervous system
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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