gata4 and gata6 expression in embryos in which Bmp signaling was blocked at 22 and 26 hpf. Embryos obtained from outcrossing a hemizygous Tg(hsp70l:dnBmpr-GFP) zebrafish were heat shocked at 22 (A-B,F-H) or 26 (C-E,I-K) hpf, harvested at 33 (A-B,F-H) or 36 (C-E,I-K) hpf, and examined for gata4 (A-E) and gata6 (F-K) expression. The first column shows wild-type siblings; the second and third columns show hemizygous embryos. The percentage of hemizygous embryos exhibiting a similar expression pattern is indicated in the lower left-hand corner (n=8-10). The expression of gata4 and gata6 in the liver region of the hemizygous embryos heat shocked at 22 hpf was reduced to a greater extent than that in the embryos heat shocked at 26 hpf. gata4 expression in the hemizygous embryos heat shocked at 26 hpf was still reduced compared with wild-type siblings, whereas gata6 expression in those embryos appeared barely affected (C-E versus I-K, arrows). Arrows point to the liver region. All images are dorsal views, anterior left.
|