FIGURE
Fig. 3
- ID
- ZDB-FIG-050721-8
- Publication
- Qian et al., 2005 - Microarray analysis of zebrafish cloche mutant using amplified cDNA and identification of potential downstream target genes
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Fig. 3
Verification of target clones by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). One microgram of total RNA sample (20 embryos each) derived from the wild-type sibling (lanes 1 and 3) and clo mutant embryos (lanes 2 and 4) were reverse transcribed and subjected to PCR analysis with specific primers corresponding to each of the perspective target genes. PCR products were electrophoresed on 1% agarose gel and stained with ethidium bromide. e1-globin and elf1a were used as internal controls. |
Expression Data
| Genes: | |
|---|---|
| Fish: | |
| Anatomical Term: | |
| Stage: | 14-19 somites |
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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