CRISPR

CRISPR1-dazl

ID

ZDB-CRISPR-220126-8

Name

CRISPR1-dazl

Previous Names

None

Target

dazl (1)

Sequence

5' - GATGGTGGGGCCAGGCCTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ae34	dazl
ae57	dazl

Expression

Gene expression in Wild Types + CRISPR1-dazl

No data available

Phenotype

Phenotype resulting from CRISPR1-dazl

No data available

Phenotype of all Fish created by or utilizing CRISPR1-dazl

Phenotype	Fish	Conditions	Figures
germ-line cyst formation disrupted, abnormal	dazl^ae57/ae57	standard conditions	Fig. 4 , Fig. 8 from Bertho et al., 2021
germ line cell increased size, abnormal	dazl^ae57/ae57	standard conditions	Fig. 4 from Bertho et al., 2021
germ line cell dazl expression decreased amount, abnormal	dazl^ae57/ae57	standard conditions	Fig. 3 from Bertho et al., 2021
germ line cell cytoplasm dazl expression decreased amount, abnormal	dazl^ae57/ae57	standard conditions	Fig. 10 from Bertho et al., 2021
germ line cell nanos2 expression decreased amount, abnormal	dazl^ae57/ae57	standard conditions	Fig. 10 from Bertho et al., 2021
germ line cell plasma membrane increased thickness, abnormal	dazl^ae57/ae57	standard conditions	Fig. 4 from Bertho et al., 2021
whole organism lacks all parts of type germ line cell, abnormal	dazl^ae57/+; dazl^mu12/+	standard conditions	Fig. 9 from Bertho et al., 2021
whole organism lacks all parts of type gamete, abnormal	dazl^ae57/+; dazl^mu12/+	standard conditions	Fig. 9 from Bertho et al., 2021
male organism male sterile, abnormal	dazl^ae57/+; dazl^mu12/+	standard conditions	Fig. 9 from Bertho et al., 2021
female organism absence of anatomical entity, abnormal	dazl^ae57/+; dazl^mu12/+	standard conditions	Fig. 9 from Bertho et al., 2021
gonad lacks all parts of type germ line cell, abnormal	dazl^ae57/+; dazl^mu12/+	standard conditions	Fig. 9 from Bertho et al., 2021
germ line cell nuclear envelope morphology, abnormal	dazl^ae57/+; dazl^mu12/+	standard conditions	Fig. 5 from Bertho et al., 2021

Citations