Morpholino mediated knock down of tef1 and tef2 differentially affects the light response in PAC2 cells.

Bar diagrams showing the expression levels as measured by RTqPCR of the indicated genes under dark control (black) and after a 3-hour light pulse (white) for control morpholino (ctr MO), tef1 morpholino (tef1 MO) and tef2 morpholino (tef2 MO) treated cells. Light induced expression of per2 and lonrf (1of2) is attenuated by both tef1 and tef2 knockdown, cry5 is affected only by tef1 knockdown, and ddb2 and cry1a are affected by neither. ef1a serves as control for a non-light responsive gene. Values represent fold β-actin mRNA levels. Statistical significance of expression differences to the light control was determined by an unpaired t test and is indicated as follows: **, pd0.01; *, pd0.05.