- Title
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Anosmin-1a is required for fasciculation and terminal targeting of olfactory sensory neuron axons in the zebrafish olfactory system
- Authors
- Yanicostas, C., Herbomel, E., Dipietromaria, A., and Soussi-Yanicostas, N.
- Source
- Full text @ Mol. Cell. Endocrinol.
Accumulation of anosmin-1a in the olfactory system. (A–C) Frontal view with dorsal region to the top; (D) dorsal view with anterior to the left. Whole-mount immunodetection of anosmin-1a in a 36 (A) and 72 hpf (B–D) embryos. Arrows indicate immuno-reactive OSN axons, which have entered olfactory bulbs. Double-headed arrows indicate the dorso-ventral (D-V) and antero-posterior (A-P) axes. OB, olfactory bulb; OE, olfactory epithelium; n, nostril. Scale bar = 150 μm in A and D, 100 μm in B, and 30 μm in C. EXPRESSION / LABELING:
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Effects of anosmin-1a depletion on the differentiation of the olfactory epithelium and axon pathfinding of olfactory sensory neurons. Frontal views of living omp-tau::GFP zebrafish embryos at 24 (A–E), 48 (F–J) and 72 hpf (K–O). Wild-type embryos (A, F, K) and kal1a morphants (B–E, G–J, L–O). The same wild-type embryo (A, F, K) and kal1a morphants (B, G, L; C, H, M; D, I, N and E, J, O) were visualized at 24, 48, and 72 hpf. Dorsal is to the top, and ventral to the bottom. Large white arrow indicates tightly fasciculated OSN axons, small white arrows show defasciculated axon fibres within olfactory bulbs, red arrows indicate disorganized olfactory sensory axons and small green arrows point to dissociated olfactory sensory neurons. OE, olfactory epithelium; ON, olfactory nerve; OB, olfactory bulbs. Scale bar = 50 μm. EXPRESSION / LABELING:
PHENOTYPE:
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Effects of anosmin-1a depletion can be rescued by ectopic expression of the protein. Frontal views of 48 hpf omp-tau;;GFP embryos injected with mis-matching control kal1a MO (A), MO kal1a-insensitive kal1a RNA (B), MO kal1a (C) or omp-tau;;GFP kal1a morphant co-injected with MO kal1a-insensitive kal1a RNA (D). Dorsal is to the top, and ventral to the bottom. White arrows indicate defasciculated axon fibres within olfactory bulbs. Red arrows show disorganized olfactory sensory axons. OE, olfactory epithelium; ON, olfactory nerve; OB, olfactory bulbs. Scale bar = 50 μm. |
Effects of anosmin-1a depletion on the terminal axon pathfinding of olfactory sensory neurons within olfactory bulbs. Frontal views of calretinin accumulation in the olfactory system of wild-type (A and C) and MO kal1a-injected embryos (B and D) at 72 (A and B) and 120 hpf (C and D). White arrows indicate protoglomeruli. Red arrows show disorganized olfactory sensory axons seen within olfactory bulbs. OE, olfactory epithelium; OB, olfactory bulbs. Scale bar = 30 μm. PHENOTYPE:
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Anosmin-1a depletion impairs the differentiation of GABAergic olfactory bulb neurons. Whole mount in situ detection of GAD67 transcripts on dissected brains of 120 hpf wild-type embryo (A), MO kal1a morphant (B), and embryo injected with a control MO (C). OB, olfactory bulbs. Scale bar = 300 μm. |
Anosmin-1a depletion impairs the differentiation of dopaminergic olfactory bulb neurons. Whole mount immunodetection of Tyrosine Hydroxylase (TH) on dissected brains of 120 hpf wild-type embryo (A), and MO kal1a morphant (B). OB, olfactory bulbs. Scale bar = 100 μm. PHENOTYPE:
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Unillustrated author statements EXPRESSION / LABELING:
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Reprinted from Molecular and Cellular Endocrinology, 312(1-2), Yanicostas, C., Herbomel, E., Dipietromaria, A., and Soussi-Yanicostas, N., Anosmin-1a is required for fasciculation and terminal targeting of olfactory sensory neuron axons in the zebrafish olfactory system, 53-60, Copyright (2009) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.