TALEN

TALEN4-pitx2

ID
ZDB-TALEN-181024-1
Name
TALEN4-pitx2
Previous Names
  • TLEN4-pitx2
Target
Target Sequence 1
5' - CGAAACACAGGCTGGA - 3'
ZFIN BLAST NCBI BLAST Ensembl
Target Sequence 2
5' - CTGGACTGGAGGTGTC - 3'
ZFIN BLAST NCBI BLAST Ensembl
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
Targets the pitx2c isoform of pitx2.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
ups6 pitx2
Expression
Gene expression in Wild Types + TALEN4-pitx2
No data available
Phenotype
Phenotype resulting from TALEN4-pitx2
No data available
Phenotype of all Fish created by or utilizing TALEN4-pitx2
Phenotype Fish Conditions Figures
atrium increased size, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
heart contraction arrhythmic, abnormal pitx2ups6/ups6 standard conditions Fig. 2,  Fig. 4 with image,  Fig. 5 from Collins et al., 2019
presumptive ventral mesoderm eve1 expression increased distribution, abnormal pitx2ups6/ups6 standard conditions Fig. 2 with image from Collins et al., 2018
whole organism vangl2 expression decreased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 7-S3 from Collins et al., 2018
mediolateral intercalation decreased process quality, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2018
whole organism anterior-posterior axis decreased length, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image,  Fig. 3 with image from Collins et al., 2018
somite shape, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2018
whole organism wdpcp expression decreased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 7-S3 from Collins et al., 2018
shield noto expression increased distribution, abnormal pitx2ups6/ups6 standard conditions Fig. 2 with image from Collins et al., 2018
notochord decreased distance prechordal plate, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2018
whole organism wnt5b expression decreased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 7-S3 from Collins et al., 2018
notochord cell cuboid, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2018
heart contraction arrhythmic, abnormal pitx2ups6/ups6 heat shock Fig. 5 from Collins et al., 2019
atrium fibrotic, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
dorsal convergence decreased process quality, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2018
atrium damaged, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
cardiac ventricle heart contraction decreased spatial extent of a process, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
forerunner cell group cell migration process quality, abnormal pitx2ups6/ups6 standard conditions Fig. 2 with image from Collins et al., 2018
atrium ppargc1a expression decreased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2019
convergent extension process quality, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image,  Fig. 3 with image from Collins et al., 2018
atrium mitochondrion morphology, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
presumptive paraxial mesoderm tbx16 expression spatial pattern, abnormal pitx2ups6/ups6 standard conditions Fig. 2 with image from Collins et al., 2018
heart contraction arrhythmic, abnormal pitx2ups6/ups6 heat shock, chemical treatment: NAC Fig. 5 from Collins et al., 2019
atrium necrotic, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
whole organism itgb1b expression increased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 7 with image from Collins et al., 2018
heart contraction decreased rate, abnormal pitx2ups6/ups6 standard conditions Fig. 2 from Collins et al., 2019
atrium cardiac myofibril disorganized, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
atrium adipor2 expression increased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2019
whole organism cxcl12b expression decreased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 7 with image from Collins et al., 2018
atrium foxo1a expression decreased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2019
atrium esrra expression increased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2019
atrium mitochondrion aggregated, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2019
heart decreased functionality, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
atrium zbtb16b expression increased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2019
atrium fabp4a expression increased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2019
atrium hypertrophic, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
atrium Z disc morphology, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
somite morphology, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2018
notochord noto expression spatial pattern, abnormal pitx2ups6/ups6 standard conditions Fig. 2 with image,  Fig. 3 with image from Collins et al., 2018
somite medial-lateral axis increased width, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2018
somite myod1 expression spatial pattern, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2018
endoderm sox17 expression spatial pattern, abnormal pitx2ups6/ups6 standard conditions Fig. 2 with image from Collins et al., 2018
atrium ucp1 expression decreased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2019
atrium M band morphology, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
somite anterior-posterior axis decreased width, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2018
forerunner cell group mislocalised, abnormal pitx2ups6/ups6 standard conditions Fig. 2 with image from Collins et al., 2018
atrium prdx5 expression decreased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2019
atrium cardiac myofibril irregular spatial pattern, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
atrium pparaa expression decreased amount, abnormal pitx2ups6/ups6 standard conditions Fig. 3 with image from Collins et al., 2019
cardiac ventricle decreased functionality, abnormal pitx2ups6/ups6 standard conditions Fig. 1 with image from Collins et al., 2019
regulation of atrial cardiac muscle cell membrane depolarization increased duration, abnormal pitx2ups6/ups6 standard conditions Fig. 2 from Collins et al., 2019
heart contraction decreased rate, abnormal pitx2ups6/+ standard conditions Fig. 2 from Collins et al., 2019
heart contraction arrhythmic, abnormal pitx2ups6/+ standard conditions Fig. 2 from Collins et al., 2019
regulation of atrial cardiac muscle cell membrane depolarization increased duration, abnormal pitx2ups6/+ standard conditions Fig. 2 from Collins et al., 2019
cell migration involved in mesendoderm migration process quality, abnormal pitx2ups6/ups6; s870Tg standard conditions Fig. 4 with image from Collins et al., 2018
extracellular matrix organization process quality, abnormal pitx2ups6/ups6; s870Tg standard conditions Fig. 7 with image from Collins et al., 2018
endoderm extracellular matrix ab2-fn labeling decreased amount, abnormal pitx2ups6/ups6; s870Tg standard conditions Fig. 7 with image from Collins et al., 2018
endodermal cell movement quality, abnormal pitx2ups6/ups6; s870Tg standard conditions Fig. 4 with image from Collins et al., 2018
endoderm anterior region increased width, abnormal pitx2ups6/ups6; s870Tg standard conditions Fig. 4 with image from Collins et al., 2018
fibronectin fibril organization decreased process quality, abnormal pitx2ups6/ups6; s870Tg standard conditions Fig. 7 with image from Collins et al., 2018
atrium sarcomere disorganized, abnormal pitx2ups6/ups6; s974Tg standard conditions Fig. 4 with image from Collins et al., 2019
atrium sarcomere morphology, abnormal pitx2ups6/ups6; s974Tg standard conditions Fig. 4 with image from Collins et al., 2019
Citations
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