PUBLICATION

Dynamic spatiotemporal coordination of neural stem cell fate decisions occurs through local feedback in the adult vertebrate brain

Authors
Dray, N., Mancini, L., Binshtok, U., Cheysson, F., Supatto, W., Mahou, P., Bedu, S., Ortica, S., Than-Trong, E., Krecsmarik, M., Herbert, S., Masson, J.B., Tinevez, J.Y., Lang, G., Beaurepaire, E., Sprinzak, D., Bally-Cuif, L.
ID
ZDB-PUB-210407-76
Date
2021
Source
Cell Stem Cell   28(8): 1457-1472.e12 (Journal)
Registered Authors
Bally-Cuif, Laure
Keywords
Notch signaling, adult neural stem cell, analytical modeling, intrinsic niche, pallium, population behavior, quiescence, spatial statistics, spatiotemporal homeostasis, zebrafish
MeSH Terms
  • Animals
  • Brain
  • Cell Proliferation
  • Feedback
  • Neural Stem Cells*
  • Neurogenesis*
  • Zebrafish
(all 7)
PubMed
33823144 Full text @ Cell Stem Cell
Abstract
Neural stem cell (NSC) populations persist in the adult vertebrate brain over a lifetime, and their homeostasis is controlled at the population level through unknown mechanisms. Here, we combine dynamic imaging of entire NSC populations in their in vivo niche over several weeks with pharmacological manipulations, mathematical modeling, and spatial statistics and demonstrate that NSCs use spatiotemporally resolved local feedback signals to coordinate their decision to divide in adult zebrafish brains. These involve Notch-mediated short-range inhibition from transient neural progenitors and a dispersion effect from the dividing NSCs themselves exerted with a delay of 9-12 days. Simulations from a stochastic NSC lattice model capturing these interactions demonstrate that these signals are linked by lineage progression and control the spatiotemporal distribution of output neurons. These results highlight how local and temporally delayed interactions occurring between brain germinal cells generate self-propagating dynamics that maintain NSC population homeostasis and coordinate specific spatiotemporal correlations.
Genes / Markers
Figures
Figure Gallery (7 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    gy2TgTransgenic Insertion
      ip11TgTransgenic Insertion
        mi2001TgTransgenic Insertion
          nns17TgTransgenic Insertion
            ups3TgTransgenic Insertion
              w2
                		Point Mutation
                1 - 7 of 7
                Show
                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Fish
                Antibodies
                Orthology
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                GFPEFGGFP
                TomatoEFGTomato
                VenusEFGVenus
                1 - 4 of 4
                Show
                Mapping
                Your Input Welcome
                We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
                Citation
                Dray, N., Mancini, L., Binshtok, U., Cheysson, F., Supatto, W., Mahou, P., Bedu, S., Ortica, S., Than-Trong, E., Krecsmarik, M., Herbert, S., Masson, J.B., Tinevez, J.Y., Lang, G., Beaurepaire, E., Sprinzak, D., Bally-Cuif, L. (2021) Dynamic spatiotemporal coordination of neural stem cell fate decisions occurs through local feedback in the adult vertebrate brain. Cell Stem Cell. 28(8):1457-1472.e12.