PUBLICATION
The RNA Binding Protein Igf2bp1 Is Required for Zebrafish RGC Axon Outgrowth In Vivo
- Authors
- Gaynes, J.A., Otsuna, H., Campbell, D.S., Manfredi, J.P., Levine, E.M., Chien, C.B.
- ID
- ZDB-PUB-150903-1
- Date
- 2015
- Source
- PLoS One 10: e0134751 (Journal)
- Registered Authors
- Campbell, Douglas, Chien, Chi-Bin, Gaynes, John, Otsuna, Hideo
- Keywords
- Axons, Retinal ganglion cells, Embryos, Zebrafish, Retina, Messenger RNA, Superior colliculus, Eyes
- MeSH Terms
-
- Actins/physiology
- Animals
- Axons/physiology*
- Gene Knockdown Techniques
- RNA-Binding Proteins/physiology*
- Retinal Ganglion Cells/physiology*
- Zebrafish/growth & development
- Zebrafish/physiology
- Zebrafish Proteins/physiology*
- PubMed
- 26325373 Full text @ PLoS One
Citation
Gaynes, J.A., Otsuna, H., Campbell, D.S., Manfredi, J.P., Levine, E.M., Chien, C.B. (2015) The RNA Binding Protein Igf2bp1 Is Required for Zebrafish RGC Axon Outgrowth In Vivo. PLoS One. 10:e0134751.
Abstract
Attractive growth cone turning requires Igf2bp1-dependent local translation of β-actin mRNA in response to external cues in vitro. While in vivo studies have shown that Igf2bp1 is required for cell migration and axon terminal branching, a requirement for Igf2bp1 function during axon outgrowth has not been demonstrated. Using a timelapse assay in the zebrafish retinotectal system, we demonstrate that the β-actin 3'UTR is sufficient to target local translation of the photoconvertible fluorescent protein Kaede in growth cones of pathfinding retinal ganglion cells (RGCs) in vivo. Igf2bp1 knockdown reduced RGC axonal outgrowth and tectal coverage and retinal cell survival. RGC-specific expression of a phosphomimetic Igf2bp1 reduced the density of axonal projections in the optic tract while sparing RGCs, demonstrating for the first time that Igf2bp1 is required during axon outgrowth in vivo. Therefore, regulation of local translation mediated by Igf2bp proteins may be required at all stages of axon development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping