PUBLICATION

T-lymphoblastic lymphoma cells express high levels of BCL2, S1P1, and ICAM1, leading to a blockade of tumor cell intravasation

Authors
Feng, H., Stachura, D.L., White, R.M., Gutierrez, A., Zhang, L., Sanda, T., Jette, C.A., Testa, J.R., Neuberg, D.S., Langenau, D.M., Kutok, J.L., Zon, L.I., Traver, D., Fleming, M.D., Kanki, J.P., and Look, A.T.
ID
ZDB-PUB-101027-8
Date
2010
Source
Cancer Cell   18(4): 353-366 (Journal)
Registered Authors
Feng, Hui, Gutierrez, Alejandro, Jette, Cicely A., Kanki, John, Langenau, David, Look, A. Thomas, Traver, David, White, Richard M., Zon, Leonard I.
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Autophagy
  • Blood Vessels/enzymology
  • Blood Vessels/pathology*
  • Cell Aggregation
  • Cell Line, Tumor
  • Cell Movement
  • Disease Progression
  • Enzyme Activation
  • Gene Expression Regulation, Leukemic
  • Humans
  • Immunohistochemistry
  • Intercellular Adhesion Molecule-1/genetics
  • Intercellular Adhesion Molecule-1/metabolism*
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/enzymology
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/genetics
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/metabolism*
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/pathology*
  • Proto-Oncogene Proteins c-akt/metabolism
  • Proto-Oncogene Proteins c-bcl-2/genetics
  • Proto-Oncogene Proteins c-bcl-2/metabolism*
  • Proto-Oncogene Proteins c-myc/metabolism
  • Receptors, Lysosphingolipid/antagonists & inhibitors
  • Receptors, Lysosphingolipid/genetics
  • Receptors, Lysosphingolipid/metabolism*
  • Zebrafish
PubMed
20951945 Full text @ Cancer Cell
Abstract
The molecular events underlying the progression of T-lymphoblastic lymphoma (T-LBL) to acute T-lymphoblastic leukemia (T-ALL) remain elusive. In our zebrafish model, concomitant overexpression of bcl-2 with Myc accelerated T-LBL onset while inhibiting progression to T-ALL. The T-LBL cells failed to invade the vasculature and showed evidence of increased homotypic cell-cell adhesion and autophagy. Further analysis using clinical biopsy specimens revealed autophagy and increased levels of BCL2, S1P1, and ICAM1 in human T-LBL compared with T-ALL. Inhibition of S1P1 signaling in T-LBL cells led to decreased homotypic adhesion in vitro and increased tumor cell intravasation in vivo. Thus, blockade of intravasation and hematologic dissemination in T-LBL is due to elevated S1P1 signaling, increased expression of ICAM1, and augmented homotypic cell-cell adhesion.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping