Morpholino

MO2-sp5l

ID

ZDB-MRPHLNO-050607-2

Name

MO2-sp5l

Previous Names

spr2-MO2 (1)

Target

sp5l (1)

Sequence

5' - CCCCCTTACACAGCCAGGTGCGTAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO2-sp5l

Expressed Gene	Anatomy	Figures
atoh1a	otic placode	Fig. 12 from Tan et al., 2022
egr2b	neural plate rhombomere 3 rhombomere 5 unspecified	Fig. 1 from Sun et al., 2006 Fig. 5 from Weidinger et al., 2005
emx3	unspecified	Fig. 5 from Weidinger et al., 2005
fezf2	neural keel	Fig. 1 from Sun et al., 2006
fgf3	axial mesoderm neural plate	Fig. 6 from Sun et al., 2006
fgf8a	neural plate tail bud	Fig. 6 from Sun et al., 2006
fgfr3	unspecified	Fig. 5 from Weidinger et al., 2005
gbx2	unspecified	Fig. 5 from Weidinger et al., 2005
her5	unspecified	Fig. 5 from Weidinger et al., 2005
hoxa2b	unspecified	Fig. 5 from Weidinger et al., 2005
hoxb1b	neuroectoderm	Fig. 1 from Sun et al., 2006
hoxb4a	unspecified	Fig. 5 from Weidinger et al., 2005
lef1	unspecified	Fig. 5 from Weidinger et al., 2005
noto	unspecified	Fig. 4 from Weidinger et al., 2005
otx1	neural plate	Fig. 1 from Sun et al., 2006
pax2a	unspecified	Fig. 5 from Weidinger et al., 2005
sp5l	otic vesicle	Fig. 10 from Tan et al., 2022
tbx16l	tail bud	Fig. 6 from Thorpe et al., 2005

Phenotype

Phenotype resulting from MO2-sp5l

Phenotype	Fish	Figures
otic placode atoh1a expression decreased amount, abnormal	AB + MO2-sp5l	Fig. 12 from Tan et al., 2022
otic placode atoh1a expression decreased distribution, abnormal	AB + MO2-sp5l	Fig. 12 from Tan et al., 2022

Phenotype of all Fish created by or utilizing MO2-sp5l

Phenotype	Fish	Conditions	Figures
otic vesicle neurog1 expression decreased amount, abnormal	AB + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic vesicle sp5l expression amount, ameliorated	AB + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic vesicle pax5 expression amount, ameliorated	AB + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic placode atoh1a expression decreased distribution, abnormal	AB + MO2-sp5l	standard conditions	Fig. 12 from Tan et al., 2022
otic vesicle pax5 expression spatial pattern, ameliorated	AB + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic vesicle sp5l expression spatial pattern, ameliorated	AB + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic placode atoh1a expression decreased amount, abnormal	AB + MO2-sp5l	standard conditions	Fig. 12 from Tan et al., 2022
otic vesicle sp5l expression amount, ameliorated	sp5a^x69/x69 + MO2-sp5l	standard conditions	Fig. 10 from Tan et al., 2022
otic vesicle sp5l expression spatial pattern, ameliorated	sp5a^x69/x69 + MO2-sp5l	standard conditions	Fig. 10 from Tan et al., 2022
otic vesicle pax5 expression spatial pattern, ameliorated	sp5a^x69/x69 + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic vesicle neurog1 expression decreased amount, abnormal	sp5a^x69/x69 + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic vesicle pax5 expression amount, ameliorated	sp5a^x69/x69 + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic placode atoh1a expression decreased distribution, abnormal	sp5a^x69/x69 + MO2-sp5l	standard conditions	Fig. 12 from Tan et al., 2022
otic placode atoh1a expression decreased amount, abnormal	sp5a^x69/x69 + MO2-sp5l	standard conditions	Fig. 12 from Tan et al., 2022
notochord truncated, abnormal	AB + MO1-wnt3a + MO2-sp5l	standard conditions	Fig. 6 from Thorpe et al., 2005
post-anal tail morphogenesis disrupted, abnormal	AB + MO1-wnt3a + MO2-sp5l	standard conditions	Fig. 6 from Thorpe et al., 2005
somite decreased amount, abnormal	AB + MO1-wnt3a + MO2-sp5l	standard conditions	Fig. 6 from Thorpe et al., 2005
post-vent region decreased length, abnormal	AB + MO1-wnt3a + MO2-sp5l	standard conditions	Fig. 6 from Thorpe et al., 2005
presumptive rhombomere 3 GFP expression decreased amount, abnormal	zf1077Tg + MO1-sp5a + MO2-sp5l	standard conditions	Fig. 6 from Labalette et al., 2015
presumptive rhombomere 4 GFP expression decreased amount, abnormal	zf1077Tg + MO1-sp5a + MO2-sp5l	standard conditions	Fig. 6 from Labalette et al., 2015
otic vesicle pax5 expression spatial pattern, ameliorated	sp5a^x69/x69; sp5l^x70/x70 + MO1-sp5a + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic vesicle sp5l expression spatial pattern, ameliorated	sp5a^x69/x69; sp5l^x70/x70 + MO1-sp5a + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic placode atoh1a expression decreased distribution, abnormal	sp5a^x69/x69; sp5l^x70/x70 + MO1-sp5a + MO2-sp5l	standard conditions	Fig. 12 from Tan et al., 2022
otic vesicle sp5l expression amount, ameliorated	sp5a^x69/x69; sp5l^x70/x70 + MO1-sp5a + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic vesicle pax5 expression amount, ameliorated	sp5a^x69/x69; sp5l^x70/x70 + MO1-sp5a + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic vesicle neurog1 expression spatial pattern, ameliorated	sp5a^x69/x69; sp5l^x70/x70 + MO1-sp5a + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic vesicle neurog1 expression amount, ameliorated	sp5a^x69/x69; sp5l^x70/x70 + MO1-sp5a + MO2-sp5l	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Fig. 10 from Tan et al., 2022
otic placode atoh1a expression decreased amount, abnormal	sp5a^x69/x69; sp5l^x70/x70 + MO1-sp5a + MO2-sp5l	standard conditions	Fig. 12 from Tan et al., 2022

Citations