PUBLICATION

The Sp1-Related Transcription Factors sp5 and sp5-like Act Downstream of Wnt/beta-Catenin Signaling in Mesoderm and Neuroectoderm Patterning

Authors
Weidinger, G., Thorpe, C.J., Wuennenberg-Stapleton, K., Ngai, J., and Moon, R.T.
ID
ZDB-PUB-050404-2
Date
2005
Source
Current biology : CB   15(6): 489-500 (Journal)
Registered Authors
Moon, Randall T., Ngai, John, Thorpe, Chris, Weidinger, Gilbert
Keywords
none
MeSH Terms
  • Animals
  • Body Patterning/physiology*
  • Cloning, Molecular
  • Cytoskeletal Proteins/metabolism
  • Ectoderm/physiology*
  • Gene Expression Regulation, Developmental*
  • Intercellular Signaling Peptides and Proteins/metabolism
  • Luciferases
  • Mesoderm/physiology*
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotides
  • Rhombencephalon/embryology
  • Signal Transduction/physiology*
  • Trans-Activators/metabolism*
  • Wnt Proteins
  • Zebrafish/embryology*
  • Zebrafish Proteins/metabolism*
  • beta Catenin
(all 18)
PubMed
15797017 Full text @ Curr. Biol.
Abstract
Background: Wnt/beta-catenin signaling regulates many processes during vertebrate development, including patterning of the mesoderm along the dorso-ventral axis and patterning of the neuroectoderm along the anterior-posterior axis during gastrulation. However, relatively little is known about Wnt target genes mediating these effects. Results: Using zebrafish DNA microarrays, we have identified several new targets of Wnt/beta-catenin signaling, including sp5-like (sp5l, previously called spr2), a zinc-finger transcription factor of the Sp1 family. sp5-like is a direct target of Wnt/beta-catenin signaling and acts together with its paralog sp5 (previously called bts1) downstream of wnt8 in patterning of the mesoderm and neuroectoderm because (1) overexpression of sp5-like, like overexpression of wnt8, posteriorizes the neuroectoderm, (2) sp5-like morpholino-mediated knockdown, like wnt8 knockdown, causes anteriorization of the hindbrain, (3) combined knockdown of sp5 and sp5-like, like loss of wnt8, causes expansion of dorsal mesoderm, (4) sp5-like knockdown reduces the defects in mesoderm and neuroectoderm patterning caused by wnt8 overexpression, and (5) inhibition of sp5-like enhances the effects of hypomorphic loss of wnt8. Importantly, (6) overexpression of sp5-like is able to partially restore normal hindbrain patterning in wnt8 morphants. Conclusions:sp5-like is a direct target of Wnt/beta-catenin signaling during gastrulation and, together with sp5, acts as a required mediator of the activities of wnt8 in patterning the mesoderm and neuroectoderm. We conclude that sp5 transcription factors mediate the downstream responses to Wnt/beta-catenin signaling in several developmental processes in zebrafish.
Genes / Markers
Marker Marker Type Name
aldh1a2GENEaldehyde dehydrogenase 1 family, member A2
axin2GENEaxin 2 (conductin, axil)
cbsbGENEcystathionine beta-synthase b
cyp26a1GENEcytochrome P450, family 26, subfamily A, polypeptide 1
dkk1bGENEdickkopf WNT signaling pathway inhibitor 1b
egr2bGENEearly growth response 2b
emx3GENEempty spiracles homeobox 3
ephb3aGENEeph receptor B3a
fgf3GENEfibroblast growth factor 3
fgfr3GENEfibroblast growth factor receptor 3
1 - 10 of 31
Show
Figures
No images available
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
w26TgTransgenic Insertion
    w34TgTransgenic Insertion
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      sp5aMO1-sp5aMRPHLNO
      sp5lMO2-sp5lMRPHLNO
      wnt8aMO1-wnt8aMRPHLNO
      wnt8aMO2-wnt8aMRPHLNO
      1 - 4 of 4
      Show
      Fish
      Fish
      w26Tg (AB)
      w34Tg
      WT
      WT + MO1-sp5a
      WT + MO1-sp5a + MO2-sp5l
      WT + MO1-wnt8a + MO2-sp5l + MO2-wnt8a
      WT + MO1-wnt8a + MO2-wnt8a
      WT + MO2-sp5l
      1 - 8 of 8
      Show
      Antibodies
      Orthology
      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      1 - 1 of 1
      Show
      Mapping
      Your Input Welcome
      We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
      Citation
      Weidinger, G., Thorpe, C.J., Wuennenberg-Stapleton, K., Ngai, J., and Moon, R.T. (2005) The Sp1-Related Transcription Factors sp5 and sp5-like Act Downstream of Wnt/beta-Catenin Signaling in Mesoderm and Neuroectoderm Patterning. Current biology : CB. 15(6):489-500.