Figure 5

Figure 5

Loss of Mfap4 alters myeloid haematopoiesis in zebrafish. (a) Schematic of the primitive wave of zebrafish haematopoiesis (reviewed in^47,48). (b–e) Expression of markers specific to the primitive wave in 10–14 somite stage WT (mfap4^+/+) or mutant (mfap4^∆/∆) embryos relative to the average expression of housekeeping genes (actb1 and eukaryotic translation elongation factor 1 alpha 1, like 1 (eef1a1)). In mutants, gata1a expression is significantly reduced (P = 0.000157; Student’s t-test), while expression of spi1b and lpc1 (P = 0.000147 and P = 0.000515, respectively; Student’s t-test) are increased. Expression of mpx, gata2a (P = 0.7 for both; Mann–Whitney-U test), lmo2 and tal1 (P = 0.855612 and P = 0.254594, respectively; Student’s t-test) are not significantly affected by loss of Mfap4. (f) Schematic of the definitive wave of zebrafish haematopoiesis (reviewed in^47,48). (g–j) Expression of markers specific to the definitive wave in 7-day-old WT or mutant larvae, relative to the average expression of housekeeping genes (actb1 and eef1a1). At 7 dpf, expression of gata2a, runx1 and spi1b (P = 0.001146, P = 0.02048, and P = 0.002625, respectively; Student’s t-test) is significantly reduced. Expression of gata1a at 7 dpf is unaffected by Mfap4 loss (P = 0.136085; Student’s t-test). Expression of mpeg1.1 is considerably, yet not significantly, reduced in mutants (P = 0.1; Mann–Whitney-U test). Expression of mpx is significantly increased in mutants (P = 0.022413; t-test), while ikaros and cpa5 expression is significantly reduced (P = 0.001643 and P = 0.000204, respectively; Student’s t-test). Error bars represent the standard error of the mean (s.e.m.) of technical triplicates of twenty pooled embryos/larvae per genotype per time point. Abbreviations: HSC, hematopoietic stem cell; CMP, common myeloid progenitor; MEP, megakaryocyte/erythroid progenitor; GMP, granulocyte/macrophage progenitor. Graphs in panels (b–e) and (g–j) were generated with Microsoft Excel (for Mac 2011, version 14.7.7). This figure was created with Inkscape software version 0.92 (available at https://www.inkscape.org/).