ZFIN Image: Barske et al., 2018, Fig. 5

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Figure Caption

Fig. 5

Edn1 Inhibits Nr2f Expression in the Mandibular Prominence

(A) edn1 is expressed comparably in the ventral ectoderm of wild-type controls and mutants at 20 hpf (arrowheads) and 30 hpf (magenta).

(B) Expression of the Edn1 receptors is reduced (ednraa) or largely unaffected (ednrab) in Nr2f mutants. Arch NCCs are labeled with dlx2a (green), and nr2f1b genotypes are indicated.

(C) In edn1 mutants, expression of all four Nr2f genes (magenta) ectopically increases to varying degrees in the mandibular domain (white arrows). Misexpression of edn1 induced by a heat shockof hsp70l:Gal4; UAS:Edn1 embryos from 20 to 24 hpf reduced Nr2f expression throughout the arches. Dashed lines reflect arch boundaries. Images are maximum-intensity projections. Merged images with dlx2a arch NCC staining are presented in Figure S5.

Scale bars, (A, top) 100 μm, (A, bottom, B, C) 20 μm.

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Acknowledgments

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Reprinted from Developmental Cell, 44(3), Barske, L., Rataud, P., Behizad, K., Del Rio, L., Cox, S.G., Crump, J.G., Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw, 337-347.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell