ZFIN Image: Wei et al., 2016, Fig. 5

Image

Figure Caption

Fig. 5

Knockdown of Pol III components polr3h and polr3k leads to HSPC defects. (A) qRT-PCR result shows that, compared with control embryos, products of Pol III (pre-tRNA^Ile, pre-tRNA^Leu, 5S rRNA and 7SL RNA) are all significantly decreased in both polr3h and polr3k morphants (5dpf). 18S rRNA was used as internal control. (B) WISH result showing that HSPC marker (cmyb) and differentiated hematopoietic cell lineage markers (gata1, pu.1 and rag1) are all decreased in polr3h and polr3k morphants at 5dpf. (C) qRT-PCR result reveals that cmyb, gata1 and pu.1 are significantly decreased in polr3h and polr3k morphants at 5dpf. β-actin was used as internal control. Note that arrowheads in B mark the CHT or the thymus. Values are mean±s.d. **P<0.01, ***P<0.001, Student′s t-test.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development