ZFIN Image: Lane et al., 2012, Fig. 7

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Figure Caption

Fig. 7 The combined knockdown of Otx and Mitf transcription factors creates a more severe phenotype.

(A) The effect of otx1a/otx2 knockdown was compared between wildtype, mitfa^w2 and mitfb^hu3857 single mutants, and mitfb^hu3857;mitfa^w2 double mutants. Morpholinos were injected at a total concentration of 2 ng/embryo using the same needle for all genotypes. Results from one representative experiment are shown. The percentage of severe RPE defects was significantly increased (P<0.0001) in the mitfb^hu3857;mitfa^w2 double mutant background compared to wildtype and single mitfa and mitfb mutants. Wildtype, N = 252; mitfa, N = 196; mitfb, N = 158; mitfa;mitfb, N = 170. (B,C) At 24 hpf dct expression is nearly eliminated in mitfb^hu3857;mitfa^w2 embryos injected with the otx1a/otx2 morpholino combination (C) compared to sibling embryos injected with control morpholino (B).

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Expression / Labeling details

Acknowledgments

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