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Fig. 4 Otx knockdown causes a decrease in dct expression in the developing RPE.
dopachrome tautomerase (dct) mRNA expression was analyzed through in situ hybridization at 24 hpf in otx morphants. dct expression was reduced only in the ventral most region of the optic cup in single morphants (B–D, white arrows) compared to controls (A). dct expression was significantly decreased in the ventral RPE of otx1a/otx2 morphants and to an even greater degree in otx1a/otx1b/otx2 morphants (E–F, white arrows). dct expression in neural crest melanocytes was unaffected (black arrows). Approximately 50 larvae were examined for each condition.