FIGURE

Fig. 6

ID

ZDB-FIG-240607-11

Publication

Lu et al., 2024 - Developmental dopaminergic signaling modulates neural circuit formation and contributes to autism spectrum disorder (ASD)-related phenotypes

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Fig. 6

Impact of a dopamine antagonist on dopaminergic and serotonergic neuron specification in zebrafish brain at 72 hours after fertilization. A and B: Tyrosine hydroxylase (TH) immunostaining in the control group (A) and in the dopamine antagonist–treated group (B). C: Quantification of TH-positive neurons in the diencephalic dopaminergic cluster (DDC). Significant differences in the normalized area of TH-positive neurons in the DDC were observed between the control and SCH 23390–treated groups (df = 12) using an unpaired t-test. D and E: 5-hydroxytryptamine (5-HT) immunostaining in the control group (D) and in the dopamine antagonist–treated group (E). F: Normalized quantification of 5-HT neurons in the raphe. Significant differences in the normalized area of serotonin (5-HT)-positive neurons in the raphe nucleus were observed between the control and SCH 23390–treated groups (df = 8). ∗P < 0.05. Scale bar = 100 μm. Ctrl, control.

Expression Data

Antibodies:	Ab2-serotonin Ab4-th
Fish:	AB
Condition:	chemical treatment by environment: SCH 23390
Anatomical Terms:	diencephalon dopaminergic neuron raphe nucleus
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical treatment by environment: SCH 23390
Observed In:	diencephalon dopaminergic neuron raphe nucleus
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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