Figure 1—figure supplement 1.
- ID
- ZDB-FIG-240603-96
- Publication
- Spikol et al., 2024 - Genetically defined nucleus incertus neurons differ in connectivity and function
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gsc2 neurons localize to the nucleus incertus. (A, A’) WISH for gsc2 and (B-C’) double-label WISH for (B, B’) gsc2 and sst1.1 or (C, C’) gsc2 and rln3a was performed on (A-B’) 4 days post-fertilization (dpf) or (C, C’) 6 dpf larvae. (A, C, C’) Dorsal views, anterior to the top. (A’, B, B’) Lateral views, anterior left. (B’, C’) Enlarged views of boxed regions in B and C, respectively. Scale bars, 100 µm. (D–F) Fluorescent double-label WISH for (D) rln3a and gsc2, (E) rln3a and nmbb, and (F) rln3a and cckb. Dorsal views of 6 dpf larvae, anterior to the top. Z-projections. Scale bar, 10 µm. (G) Schematic depicting distribution of neuronal subtypes in the nucelus incertus (NI) of larval zebrafish. Green dots, gsc2 expression; purple dots, rln3a expression; blue dots, nmbb expression; pink dots and shading, cckb expression. IPN: interpeduncular nucleus, PAG: periaqueductal grey, NI: nucleus incertus. |