Fig. 2

LMY alleviates HCF-induced hepatic lipid accumulation in miceQuantification of hepatic TG (A), TC (B), NEFA (C), MDA (D), blood glucose (E), HOMA-IR (F), and serum ALT (G) and AST (H) levels in HF-related food-induced NAFLD mice treated with or without LMY. Normal diet-fed mice were used as blank controls. (I) Typical H&E staining images of the liver of blank, HF-fed, and LMY-treated mice. C57BL/6 mice were randomly selected at the age of 10 weeks. The HF diet was given to the mice for 6 weeks, except the blank group, which was subjected to LMY treatment for another 6 weeks. The serum of these treated mice was harvested for ALT and AST measurements, while their livers were dissected for TG, TC, NEFA, and MDA quantification, as well as paraffin sections for HE staining. Six individuals were included in each treatment group (n=6). All these experiments were conducted three times in a double-blind manner, and the quantification results are presented as the mean±SEM. ***P<0.001, and ****P<0.0001. ns, no significance.