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Fig. 1

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ZDB-FIG-240523-70

Publication

Zhang et al., 2024 - Lian-Mei-Yin formula alleviates diet-induced hepatic steatosis by suppressing Yap1/FOXM1 pathway-dependent lipid synthesis

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Fig. 1

LMY alleviates HCF-induced hepatic lipid accumulation in zebrafish(A) Scheme of HCF diet induction and drug treatments for studying the time course and dose effect of LMY in larval zebrafish. (B) Typical oil red O staining images showing the alleviation of lipid accumulation over time by LMY in larval zebrafish. The number of zebrafish larvae involved in each experimental group is indicated in the top left corner of each image. (C) Typical oil red O staining images showing the dose-dependent alleviation of lipid accumulation by LMY in larval zebrafish. The number of zebrafish larvae involved in each experimental group is indicated in the top left corner of each image. (D) Relative quantification of the time course through which lipid accumulation alleviated the effect of LMY in larval zebrafish. The average oil red O staining intensity of HCF-fed zebrafish on M6D was defined as 100%. (E) Quantification of the dose-dependent alleviation of lipid accumulation by LMY in zebrafish. The average oil red O staining intensity of HCF-fed zebrafish was defined as 100%. (F) Quantification of the liver TG concentration in adult zebrafish treated with different doses of LMY or NK-104. (G) Quantification of the liver MDA concentration (TBARS value) in adult zebrafish treated with different doses of LMY or NK-104. (H) Quantification of the HOMA-IR index in adult zebrafish treated with different doses of LMY. For TG, MDA, and HOMA-IR analysis, adult zebrafish were randomly selected at the age of 12 weeks postfertilization (wpf) and were administered the HCF diet for four weeks, except the blank group. HCF-diet-induced zebrafish were then treated with different doses of LMY or NK104 for another four weeks. The livers of these zebrafish were then harvested for TG, MDA, and HOMA-IR analysis. Six individuals were included in each experimental group (n=6). All these experiments were conducted three times in a double-blind manner, and the quantification results are presented as the mean±SEM. *P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001. ns, no significance.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Acta. Biochim. Biophys. Sin (Shanghai)