FIGURE

Fig. 4

ID

ZDB-FIG-240522-16

Publication

Madakashira et al., 2024 - DNA hypomethylation activates Cdk4/6 and Atr to induce DNA replication and cell cycle arrest to constrain liver outgrowth in zebrafish

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Fig. 4

Cdk4/6 inhibition by Palbociclib (PD) rescues abnormal cell cycle gene expression in uhrf1 mutants. (A) PCA analysis of RNA Seq from 120 hpf Palbociclib (PD) treated and DMSO treated uhrf1−/− and control livers. (B) Venn diagram of differential expressed genes (DEGs) (cutoff P-adj < 0.05) between uhrf1−/− and their control siblings treated with DMSO and PD. (C) Unsupervised clustering heatmap of the expression profile of 120 hpf DMSO and PD treated uhrf1−/− liver. z-scores based on raw counts. Rows are divided into 10 clusters calculated on the hierarchical clustering of dendrogram (Euclidean distance). (D) REVIGO gene ontology of cluster 4 and (E) and cluster 5. (F) Key cell cycle genes, immune and tnfa response gene expression between PD and DMSO treated uhrf1−/− plotted as a heatmap with log2fold change. (G) IPA of the cell cycle genes differentially expressed between 120 hpf uhrf1−/− PD and DMSO livers, cell cycle checkpoint regulation pathways indicated in bold. P-value *< 0.05, **< 0.005, *** < 0.0005 by unpaired Student's t-test with adjustment for multiple comparisons and represented as median with range.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.