FIGURE

Fig. 1

ID

ZDB-FIG-240522-13

Publication

Madakashira et al., 2024 - DNA hypomethylation activates Cdk4/6 and Atr to induce DNA replication and cell cycle arrest to constrain liver outgrowth in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

uhrf1 mutant hepatocytes accumulate in late S-phase with increased DNA replication. (A) Representative Z-stack projection confocal images of EdU and Hoechst stained control and uhrf1−/− livers. Control and uhrf1−/− embryos were pulsed with EdU for 30 min at 80 hpf, 96 hpf and 120 hpf and processed for EdU fluorescence by Click-it. (B) Quantification of EdU incorporation in A. (C) 80 hpf, 96 hpf and 120 hpf hepatocytes were sorted into early, mid and late S phase based on EdU pattern and quantified. (D) Quantification of EdU incorporation from a 12 min EdU pulse at 80 hpf and 120 hpf control and uhrf1−/− hepatocytes. Pie chart represents percentage of hepatocytes in each stage of S phase. Scale bar = 50 μm, the number of samples and clutches indicated for each condition and represented as median with range. P-value *< 0.05, **< 0.005, *** < 0.0005 by unpaired Student's t-test with adjustment for multiple comparisons.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.