Fig. 7

Cold exposure induces a lipid cycling and mitochondrial biogenesis transcriptomic program in zebrafish eAT (A) Schematic of cold acclimatization of adult zebrafish. (B) PCA score plot showing clustering of epicardial adipocytes according to environmental temperature. (C) Volcano plot showing DEGs of eAT in cold-exposed compared with control siblings maintained at ambient temperature. The x axis shows log2 fold change and the y axis adjusted p value on a −log10 scale. (D) Heatmap of the top 15 most differentially upregulated and downregulated genes in the eAT of cold-acclimatized fish. (E) Top enriched pathways from KEGG and Reactome analyses of the upregulated genes (796) in cold-exposed fish. (F) Bar graph depicting cold-induced elevation of active mitochondrial content in epicardial adipocytes, measured as mean fluorescent intensity of MTDR (in arbitrary units [a.u.]). Data are presented as mean ± SEM. ∗∗∗∗p < 0.0001, two-tailed t test. n indicates the number of adipocytes from 5 control and 3 cold-treated fish.