Relative expression of rbfox family mRNAs in adult brains and 3dpf larvae from the rbfox1sa15940 line. Adult brains: Relative brain expression of (A) rbfox1, (B) rbfox1l, rbfox2, rbfox3a and rbfox3b mRNA in adult brains. mRNA expression is normalised to the average expression of the mRNA in wild-type fish and to the reference housekeeping genes ribosomal protein L13a (rpl13) (A, B) or the eukaryotic translation elongation factor 1 alpha 1a (eef1a1a) (B). Kruskal-Wallis test followed by Dunn’s multiple comparison test. n = 5-7 WT, 5-7 HZ, 5-7 HOM. Mean ± SD. C Brain expression of the rbfox family mRNAs in adult wild-type zebrafish brains represented as the quantification cycle value for each gene. n = 7 WT, 7 HZ, 7 HOM. Mean ± SD. 3dpf larvae: Relative brain expression of (D) rbfox1, (E) rbfox1l, rbfox2, rbfox3a and rbfox3b mRNA in 3 dpf whole larvae. mRNA expression is normalised to the average expression of the mRNA in wild-type fish and to the reference housekeeping gene ubiquitin-conjugating enzyme E2A (ube2a). Kruskal-Wallis test followed by Dunn’s multiple comparison test. n = 5 WT, 5 HZ, 5 HOM. Mean ± SD. F Brain expression of the rbfox family mRNAs in 3 dpf wild-type larvae represented as the quantification cycle value for each gene. n = 5 WT, 5 HZ, 5 HOM. Mean ± SD. Each point represents the results from a pool of 10 larvae.
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