FIGURE

Fig. 5

ID

ZDB-FIG-240208-41

Publication

Vaz-Rodrigues et al., 2023 - Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndrome

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Fig. 5

Validation of mRNA levels for target differentially expressed genes (DEGs) and corroboration of target differentially represented proteins (DRPs) levels, followed by summary of target results across all methods. (A) Quantification by reverse transcriptase quantitative PCR (RT-qPCR) of changes in mRNA levels of 5 target DEGs, including the 2 upregulated genes macrophage migration inhibitory factor (mif) and cathepsin S (ctss2.1), and 3 downregulated ones, namely DNA-dependent protein kinase (prkdc), von Willebrand factor (vwf) and smooth muscle alpha-actin (acta2). (B) Reliability of proteomics data was confirmed by Western blotting using antibodies against target DRPs, actin alpha 2 [UniProt ID: Q6DHS1] and 60 kDa chaperonin [UniProt ID: Q803B0] and the loading control β-tubulin. (C) Analyzed targets are represented by a color code based on fold change values and according to the applied methodology.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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