Figure 2—figure supplement 1.

Expression of tnfa, cxcr4b, cxcl12a mRNA during Salmonella infection.

RT-qPCR analysis of (A) tnfa, (B) cxcr4b, and (C) cxcl12a mRNA expression. Embryos were either injected with PBS or Sal-GFP and larvae presenting a high bacterial proliferation were excluded from the analysis. RNA samples were extracted from whole larvae at 3 hpi, 1 dpi, 2 dpi, 3 dpi, and 4 dpi. Data are presented as relative target gene/ef1a expression in the Sal-infected or PBS-injected larvae using the formula 2−ΔCp. Of note, as tnfa expression was usually not detected in PBS condition, data could not be presented as relative expression (Infected versus PBS) as for other genes. Values are the means ± SEM of eight replicates. Per replicate, eight larvae per time points were used (Mann–Whitney test, two-tailed **p < 0.01; ***p < 0.001, ns: not significant).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife