Fig. 2

Defects of retinal cell development in stmn4^−/−. (A) Expressions of retinal opn1lw1, opn1sw2, rhodopsin, and opn1mw1 at 72 hpf in WT and stmn4^−/− zebrafish larvae (A1 − A8), and the calculation of the relative expression levels in each sample (A9). (B) Expression of retinal fluorescence in Tg (Huc: EGFP) and Tg (stmn4^−/−; Huc: EGFP) at 48 hpf zebrafish embryos (B1 − B4) and 7 dpf zebrafish larvae (B6 − B9), and the quantification of relative fluorescence in each sample (B5, B10). (C) Immunofluorescence assays for the Opn1sw2, Opn1lw1 and Rhodopsin expression at 96 hpf in WT and stmn4^−/− zebrafish larvae (C1 − C8, C10 − C17, C19 − C26), and the quantification of relative fluorescence in each sample (C9, C18, C27). A1 − A8, head to the up, and dorsal to the down; B1 − B4, B6 − B9, lateral view, anterior to the left, and dorsal to the up. Scale bar, 200 μm (A1 − A8), 100 μm (B1 − B4, B6 − B9), 50 μm (C1 − C8, C10 − C17, C19 − C26), 25 μm (C7, C8, C16 − C17, C25 − C26). ***P < 0.001, **P < 0.01, *P < 0.05