Fig. 1

Stmn4 deficiency led to eye morphological defects. (A) Copper (Cu) stress induced obviously reduced expression of stmn4 in eyes during zebrafish embryogenesis (A1 − A4), cross-section of the eye (A5, A6) and the calculation of the relative expression levels of stmn4 (A7). (B) Western blot detection of Stmn4 protein levels in WT and stmn4^−/− at 24 hpf and 48 hpf (B1 − B2), and the mRNA levels of stmn4 in WT and stmn4^−/− at 24 and 48 hpf (B3), respectively. (C) Embryonic and eye development in stmn4^−/− and WT embryos and larvae at different developmental stages (C1 − C12), and the quantification of the eye size in WT and stmn4^−/− embryos and larvae (C13), respectively. (D) HE staining showed the difference in the eyes of WT and stmn4^−/−embryos at 48 hpf (D1 − D4), and the calculation data (D5, D6). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. A1, A2, lateral view, anterior to the left, and dorsal to the up; A3, A4, head to the up, and dorsal to the down; C1 − C12, lateral view, anterior to the up, and dorsal to the right. Scale bar, 200 μm (A1 − A4), 100 μm (A5, A6, C1 − C12, D1, D2), 50 μm (D3, D4). ***P < 0.001, **P < 0.01, *P < 0.05