FIGURE

FIGURE 3

ID

ZDB-FIG-231228-125

Publication

Abdel-Razek et al., 2023 - Calcium signaling mediates proliferation of the precursor cells that give rise to the ciliated left-right organizer in the zebrafish embryo

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FIGURE 3

Validation of screen results that identify Ca2+ signaling as a regulator of DFC proliferation. (A) Diagram of SERCA function to pump Ca²⁺ from the cytoplasm into endoplasmic reticulum stores, and the action of the small molecules thapsigargin (Thaps) and cyclopiazonic acid (CPA) to inhibit SERCA. Created using BioRender.com. (B) Treating embryos with 1 μM Thaps or 100 μM CPA significantly reduced the mitotic index of DFCs as compared to negative control DMSO treated embryos. Bar graphs indicate average values and error bars represent one standard deviation. Each circle on the graphs represents results from an individual embryo. An unpaired two-tailed t-test with Welch’s correction was used for statistical analysis. * = significant difference. n = number of embryos analyzed. (C) Representative merged images of pHH3 staining in Tg(sox17:EGFP-caax) embryos treated with 1% DMSO (negative control), 1 μM Thaps, or 100 μM CPA. Arrows point out mitotic DFCs and asterisks point out mitotic neighboring (non-DFC) cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Mol Biosci