FIGURE

Fig. 7

ID

ZDB-FIG-231215-83

Publication

Baker et al., 2022 - In Toto imaging of early enteric nervous system Development reveals that gut colonization is tied to proliferation downstream of ret

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Fig. 7

retwmr1/+ ENCCs display accelerated neuron immunoreactive signatures. (A) Cartoon larvae denotes foregut (Fg), midgut (Mg) and hindgut (Hg) intestine (yellow) with red box indicating foregut-midgut boundary region of interest from which representative images are displayed. (B,C,E,F) Whole-mount immunohistochemistry performed using antibodies targeting ChAT and Elavl3. Representative images reveal ChAT (magenta) and Elavl3 (cyan) expression, and their colocalized (coloc) channel (white) within gut tube (white dashed outline) at 96 hpf (B,C) and 120 hpf (E,F). Yellow arrowheads indicate co-positive ChAT+/Elavl3+ ENCCs. (D,G) Percent co-positive ChAT+/Elavl3+ calculated from total Elavl3+ enteric neurons at 96 hpf (**P=0.0083) (D) and 120 hpf (NS) (G). In D ret+/+ n=11, retwmr1/+ n=7; in G ret+/+ n=6, retwmr1/+ n=10. Data are mean±s.e.m. Scale bars: 20 µm (B,C,F); 15 µm (E).

Expression Data

Antibody:	Ab1-elavl
Fish:	ret^wmr1/+; em2Tg/em2Tg
Condition:	ultraviolet light
Anatomical Term:	enteric neuron
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ret^wmr1/+; em2Tg/em2Tg
Condition:	ultraviolet light
Observed In:	enteric neuron gut neural crest cell differentiation
Stage:	Day 4

Phenotype Detail

Acknowledgments

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