FIGURE

Fig. 3

ID

ZDB-FIG-231215-79

Publication

Baker et al., 2022 - In Toto imaging of early enteric nervous system Development reveals that gut colonization is tied to proliferation downstream of ret

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

retwmr1/+ fish display a reduction in total ENCC number and migratory extent along the gut. (A,B) Images of ret+/+ (A) and retwmr1/+ Tg(−8.3phox2bb:Kaede) (B) fish show phox2bb+ ENCCs within the intestine at 96 hpf. retwmr1/+ ENCCs fail to reach distal hindgut at 96 hpf. (C) Average number of ENCCs present during the 48-96 hpf time-lapse in ret+/+ and retwmr1/+ fish (n=3). (D,E) Snapshot of final 96 hpf time-point depicts migratory track of leading-edge cell (vanguard) in left (pink spot) and right (blue spot) ENCC chains (cloaca, white asterisk). (F) Average distance traveled by left and right vanguard cells in ret+/+ and retwmr1/+ fish compared across 48-96 hpf (n=6 per condition; data are mean±s.e.m.). (G) Final position of vanguard cells at 96 hpf (Wilcoxon signed-rank test, **P=0.0022). (H,I) Schematic depicts vanguard cells in left (pink) and right (blue) ENCC migratory chains in (H) ret+/+ and (I) retwmr1/+. Scale bars: 100 µm. A, anterior; D, dorsal; P, posterior; V, ventral.

Expression Data

Gene:	Kaede
Fish:	ret^wmr1/+; em2Tg/em2Tg
Condition:	ultraviolet light
Anatomical Term:	gut migratory neural crest cell
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ret^wmr1/+; em2Tg/em2Tg
Condition:	ultraviolet light
Observed In:	gut migratory neural crest cell gut neural crest cell migration
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development