Fig. 3.

mpeg1⁺ cells engulf axonal debris after cutaneous injury in vivo. (A) Schematic for inducing scale injury via micro scissors in vivo. (B) Confocal images from a time-lapse of in vivo micro scissor scale injury from an adult expressing reporters for somatosensory axons [Tg(p2rx3a:mCherry)] and mpeg1⁺ cells [Tg(mpeg1:NTR-EYFP)]. Solid white lines outline scales from fish, yellow dashed line oval denotes site of micro scissor injury, yellow dotted line box denotes the region magnified in B′. Yellow dotted line boxes in B′ denote the regions magnified in insets. Time denotes mm:ss relative to the time of injury. See Movies 5 and 6. (C) Schematic for laser axotomy in vivo. Representative image of axons pre- (left) and post- (right) laser axotomy. Yellow asterisk denotes site of axotomy. (D) Confocal images from a time-lapse of axon degeneration from an adult expressing reporters for keratinocyte phagosomes [Tg(krt4:EGFP-2xFYVE);TgBAC(ΔNp63:EGFP-2xFYVE)], somatosensory axons [Tg(p2rx3a:mCherry)] and mpeg1⁺ cells [Tg(mpeg1:NTR-EYFP)] before and during laser axotomy-induced axon degeneration. Arrowhead denotes engulfed axonal debris. (E) Quantification of total axon volume engulfed over time by keratinocytes and mpeg1+ cells. Two-way ANOVA followed by Bonferroni tests determined significance of differences between mpeg1⁺ cells and keratinocytes. **P<0.01. n=8 ROIs from N=3 fish. Scale bars: 100 μm (B), 20 μm (B′), 10 μm (C,D). Error bars in E represent s.d.