Figure 6

Effects of socs3b ablation on adult development and hematopoiesis. Representative images of wildtype (WT, socs3b^wt/wt) and knockout (KO, socs3b^mdu24/mdu24) zebrafish at 5 months post fertilization (mpf) (A, B), with scale bars representing 1 cm, along with quantitation of their size showing results for individual fish along with mean and SEM, with significance tested using a parametric t-tests (n = 8) (C). Gene expression analysis of the indicated genes in WT and KO adult kidney presented as fold change (log₂) relative to WT, showing mean and SEM. Normalized Cq values obtained from WT and KO kidney marrow was used to test for significance using parametric t-tests (n = 6) (D). Flow cytometry analysis of WT and KO kidney for erythrocyte, lymphocyte, myeloid cell and precursor (E, F) populations, with analysis of mpx+ neutrophils (H, I) and mpeg1.1+ macrophages (K, L) as well as quantitation of relevant populations (G, J, M) showing mean and SEM with significance tested using t-tests with Welch’s correction (n = 10) (*** p < 0.001, ** p < 0.01, * p < 0.05). Histological analysis of adult kidney (N, O) and blood (Q, R), showing representative images, as well as quantitation of both (P, S), showing mean and SEM, a parametric t-tests (n = 6 and n = 8, respectively) (F). Mb, myeloblast; Pro, promyelocyte; M, monocyte; L, lymphocyte; N, neutrophil; E, erythrocyte.