Figure 1

Generation of socs3b KO allele. Schematic of the SOCS3 protein displaying conserved functional domains (KIR: kinase inhibitory region, ESS: extended SH2 sub-domain, SH2: Src homology 2 domain, SOCS box) along with the relative conservation (% identity) of zebrafish Socs3a and Socs3b to the indicated domains (A), as well as alignment of SOCS3 with Socs3a and Socs3b sequences (B). The zebrafish socs3b gene structure, showing intron (thin line) and exons (boxes) and sequences targeted with gRNAs (C). Sequence traces of representative homozygous wildtype (WT, socs3b^wt/wt) and knockout (KO, socs3b^mdu24/mdu24) fish around the sites targeted with gRNA1 (upper) or gRNA2 (lower), including nucleotides and encoded amino acids. Nucleotide sequences deleted are boxed red and those inserted boxed purple, with de novo protein sequences shown in red (D). Schematic of Socs3b protein forms expressed in WT and KO fish (E).