Figure 7
- ID
- ZDB-FIG-230321-18
- Publication
- Xiao et al., 2023 - Dopey2 and Pcdh7 orchestrate the development of embryonic neural stem cells/ progenitors in zebrafish
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Dopey2 and Pcdh7b complement the development and arrangement of neural cells in zebrafish embryonic brains (A) Real-time fluorescence quantitative PCR analysis shows the relative expression level of sox2, huc at 48 hpf in embryos pre-inject with Ctl MO, DO MO, P7 MO, DO MO + P7 MO, DO MO + P7 mRNA, P7 mRNA, and P7 MO + DO mRNA (mean ± s.e.m, n = 3, Student’s t test: ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05). (B–E) ISH analysis of sox2, huc, olig2 and wnt1 for embryos at 48 hpf, 45 hpf and 24 hpf pre-injected with Ctl MO, DO MO, P7 MO and DO MO + P7 MO. Scale bar = 200 μm. (F) Real-time fluorescence quantitative PCR analysis shows the relative expression level of sox2, huc at 48 hpf in WT, DO MUT or P7 MUT embryos pre-inject with Ctl MO, DO MO + P7 MO (mean ± s.e.m, n = 3, Student’s t test: ∗∗p < 0.01). Ctl MO: control MO, DO MO: dopey2 MO, DO mRNA: dopey2 mRNA, P7 MO: pcdh7b MO, P7 mRNA: pcdh7b mRNA, DO MUT: dopey2mutant, P7 MUT: pcdh7bmutant. |