Figure 6

Pcdh7b modulates the differentiation of neural stem cells/progenitors and inhibits the proliferation of neural stem cells/progenitors in zebrafish embryonic brains

(A) In situ hybridization analysis detected the expression of nestin, sox2, and huc genes. Emryos were at 48 hpf, 50 hpf, pre-injected with Ctl MO, P7 MO and P7 MO + P7 mRNA. Scale bar = 200 μm.

(B) Real-time fluorescence quantitative PCR analysis showed the relative expression level of nestin, sox2, huc and olig2 gene at 48 hpf in embryos pre-injected with Ctl MO, P7 MO and P7 MO + P7 mRNA (mean ± s.e.m, n = 3, Student’s t test: ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05).

(C) Representative immunofluorescence staining detected Sox2 in the frozen sections of 24 hpf embryos pre-injected with Ctl MO or P7 MO. Scale bar = 50 μm.

(D) Whole-mount immunofluorescence staining for H3S10P in Ctl MO, P7 MO and P7 mRNA pre-injected embryos at 24 hpf. Scale bar = 100 μm.

(E) Statistical analysis of the number of H3S10P-positive cells in embryos pre-injected with Ctl MO, P7 MO and P7 mRNA. The number in the dotted box region in (A) was counted. For each group, 6 embryos were scored (mean ± s.e.m, n = 3, Student’s t test: ∗p < 0.05). Images were quantified by ImageJ software. Ctl MO: control MO, P7 MO: pcdh7b MO, P7 mRNA: pcdh7b mRNA.