FIGURE

Fig. 6

ID

ZDB-FIG-230315-113

Publication

Zhang et al., 2022 - Deficiency of nde1 in zebrafish induces brain inflammatory responses and autism-like behavior

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Fig. 6

nde1 mRNA rescue mitosis, apoptosis, and immune-related phenotype

(A–C). Relative expression level of bicd2, gmnn, pcna, pidd1, bbc3, bcl2a, and bcl2l1, apoeb in nde1^+/+, nde1^−/−, and nde1 mRNA rescued zebrafish.

(D) AO staining of nde1^+/+, nde1^−/−, and nde1 mRNA rescued zebrafish at 28 hpf. White dotted oval indicates telencephalon, midbrain, rhombomere, and spine, respectively. Scale bar: 0.2 mm.

(E) Statistics of apoptotic bodies after AO staining.

∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Data are presented as mean ± SEM

Expression Data

Genes:	apoeb bbc3 bcl2a bcl2l1 bicd2 gmnn pcna pidd1 (all 8)
Fish:	TU nde1^{zf3784/zf3784} (TU)
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Day 4

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
apoeb	TU	standard conditions	Day 4	whole organism	RTPCR
apoeb	nde1^{zf3784/zf3784} (TU)	standard conditions	Day 4	whole organism	RTPCR
bbc3	TU	standard conditions	Prim-15	whole organism	RTPCR
bbc3	nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-15	whole organism	RTPCR
bcl2a	TU	standard conditions	Prim-15	whole organism	RTPCR
bcl2a	nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-15	whole organism	RTPCR
bcl2l1	TU	standard conditions	Prim-15	whole organism	RTPCR
bcl2l1	nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-15	whole organism	RTPCR
bicd2	TU	standard conditions	Prim-5	whole organism	RTPCR
bicd2	nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-5	whole organism	RTPCR

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Antibody Labeling

No data available

Phenotype Data

Fish:	nde1^{zf3784/zf3784} (TU)
Observed In:	midbrain apoptotic process rhombomere apoptotic process spinal cord apoptotic process telencephalon apoptotic process whole organism
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Fish	Conditions	Stage	Phenotype
nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-5	midbrain apoptotic process increased process quality, abnormal
nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-5	rhombomere apoptotic process increased process quality, abnormal
nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-5	spinal cord apoptotic process increased process quality, abnormal
nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-5	telencephalon apoptotic process increased process quality, abnormal
nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-5	whole organism bicd2 expression increased amount, abnormal
nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-5	whole organism gmnn expression increased amount, abnormal
nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-5	whole organism pcna expression increased amount, abnormal
nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-15	whole organism bbc3 expression increased amount, abnormal
nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-15	whole organism bcl2a expression decreased amount, abnormal
nde1^{zf3784/zf3784} (TU)	standard conditions	Prim-15	whole organism bcl2l1 expression decreased amount, abnormal

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Acknowledgments

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