FIGURE

Fig 2

ID

ZDB-FIG-230305-20

Publication

Song et al., 2023 - Disruption of T-box transcription factor eomesa results in abnormal development of median fins in Oujiang color common carp Cyprinus carpio

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Fig 2

The efficiency of knock out at the eomesa1 and eomesa2 loci was evaluated by Sanger sequencing method at 24 hours post fertilization.

(a) The procedure of gene editing efficiency measurement on 24 hpf embryo. (b) The sequencing chromatographs of TA cloning results show the representatives of three types of mutations including insertion (+7 bp), deletion (-7 bp) and both (-5,+7 bp) around T1 of eomesa1 locus. The red rectangles indicate the target bases, and the blue rectangles indicate the inserted bases.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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