ZFIN Image: Song et al., 2023, Fig 2

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Figure Caption

Fig 2 The efficiency of knock out at the eomesa1 and eomesa2 loci was evaluated by Sanger sequencing method at 24 hours post fertilization.

(a) The procedure of gene editing efficiency measurement on 24 hpf embryo. (b) The sequencing chromatographs of TA cloning results show the representatives of three types of mutations including insertion (+7 bp), deletion (-7 bp) and both (-5,+7 bp) around T1 of eomesa1 locus. The red rectangles indicate the target bases, and the blue rectangles indicate the inserted bases.

Acknowledgments

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