FIGURE SUMMARY
Title

Disruption of T-box transcription factor eomesa results in abnormal development of median fins in Oujiang color common carp Cyprinus carpio

Authors
Song, S., Du, B., Chung-Davidson, Y.W., Cui, W., Li, Y., Chen, H., Huang, R., Li, W., Li, F., Wang, C., Ren, J.
Source
Full text @ PLoS One
Fig 1

The phylogenetic tree, amino acid alignment of Eomes in vertebrates, and the schematic diagrams of the eomesa gene structure.

(a) The phylogenetic tree was constructed using the ML method with 1,000 bootstraps. The cyan square represents teleost-specific whole genome duplication (TS-WGD, or 3R-WGD) and green triangles represent the fourth-round whole genome duplication (4R-WGD) occurred before the speciation of the common ancestor of common carp and goldfish. (b) Dashes are introduced to improve the alignment. The T-box domain is marked by colored boxes. (c) The gray boxes indicate untranslated regions. Yellow boxes indicate exons, and red boxes indicate exons encoding the T-box domain. The dash lines indicate the target sites.
Fig 2

The efficiency of knock out at the eomesa1 and eomesa2 loci was evaluated by Sanger sequencing method at 24 hours post fertilization.

(a) The procedure of gene editing efficiency measurement on 24 hpf embryo. (b) The sequencing chromatographs of TA cloning results show the representatives of three types of mutations including insertion (+7 bp), deletion (-7 bp) and both (-5,+7 bp) around T1 of eomesa1 locus. The red rectangles indicate the target bases, and the blue rectangles indicate the inserted bases.
Fig 3

The efficiency of knock out at the eomesa1 and eomesa2 loci was evaluated by Sanger sequencing method at 7 days post fertilization.

(a) The measurement procedure of gene editing efficiency includes population editing efficiency (Ep) and individual editing efficiency (Ei) on 7 dpf larvae. (b) Sanger sequencing chromatographs show overlapped peaks in the eomesa1 T2 and eomesa2 T4 sites in targeted individuals, and the ratio of peak heights from these overlapped peaks between WT and mutants implies the knockout efficiency. The red rectangles indicate the target bases.
Fig 4

Phenotypic comparison between zebrafish and color common carp eomesa mutants.

(a) Lateral view of wild type and eomesa mutant zebrafish. Compared to the wild type, the eomesafh105 mutants exhibit two phenotypes. All mutants exhibit the phenotype with no dorsal fin. Rare mutants exhibit the phenotype with no dorsal fin and anal fin. (b) Lateral view of four-month-old wild type and eomesa mosaic mutants of color common carp. Compared to the wild type, three mosaic mutants (M1-M3) exhibit different degrees of deformity in the dorsal fins and complete loss of anal fins.

PHENOTYPE:
Fish:
Observed In:
Stage: Adult
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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