FIGURE

Fig. 7

ID

ZDB-FIG-230115-64

Publication

Brandt et al., 2021 - Ablation of mpeg+ Macrophages Exacerbates mfrp-Related Hyperopia

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Fig. 7

Collagen fibril diameter in mfrp⁺^/^– and mfrp^–^/^– sclera with and without macrophage ablation. Representative examples of collagen fibrils in the central posterior sclera of mfrp⁺^/^–; mpeg1:NTR-eYFP⁺ (A) and mfrp^–^/^–; mpeg1:NTR-eYFP⁺ (A') untreated fish. (B) Frequency distribution of collagen fiber diameter with y-axis = relative frequency as a percentage and x-axis = collagen fiber diameter in 5-nm bins. mfrp⁺^/^– is indicated by a green line, mfrp^–^/^– by a purple line. For mfrp⁺^/^–, n = 3; for mfrp^–^/^–, n = 4. (C) Representative examples of collagen fibrils in the central posterior sclera of mfrp⁺^/^–; mpeg1:NTR-eYFP⁺ (C) and mfrp^–^/^–; mpeg1:NTR-eYFP⁺ (C') MTZ treated fish. (D) Frequency distribution of collagen fiber diameter with y-axis = relative frequency as a percentage and x-axis = collagen fiber diameter in 5-nm bins. mfrp⁺^/^– is represented by a blue line, mfrp^–^/^– by a yellow line. For mfrp⁺^/^–, n = 5; for mfrp^–^/^–, n = 5. Multiple t-tests were used for statistical analysis. *P < 0.001. (E) Combined graph of (B) and (D). Errors bars represent standard deviations throughout figure. Two-way ANOVA with Tukey's multiple-comparisons were used for statistical analysis. An asterisk (*) denotes a significant difference between mfrp⁺^/^– MTZ treated and mfrp^–^/^– MTZ treated; a plus sign (+) denotes a significant difference between mfrp⁺^/^– MTZ treated and mfrp⁺^/^– untreated; and the pound symbol (#) denotes a significant difference between mfrp⁺^/^– MTZ treated and mfrp^–^/^– untreated. P < 0.05 for all significant differences in (E).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mfrp^mw78/+; w202Tg/w202Tg mfrp^mw78/mw78; w202Tg/w202Tg
Condition:	chemical treatment by environment: metronidazole
Observed In:	sclera collagen fibril organization
Stage:	Days 45-89

Phenotype Detail

Acknowledgments

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